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- Title
Improvement of Yeast Strains by Mutation for Enhancement of Ethanol Tolerance.
- Authors
BHARTI, R.; HARINIKUMAR, K. M.; DOSS, D. D.; SHIVAPRAKASH, M. K.; MUTHURAJU, R.
- Abstract
The improvement of yeast strains (Saccharomyces cerevisiae) is getting more attention due to its high fermentation capacity that can be used as a renewable energy product. Uncovering genetic control of variation in ethanol tolerance in natural populations of yeast Saccharomyces cerevisiae is essential for understanding the evolution of fermentation. In order to obtain mutant strains showing higher bioethanol production than wild-type strains, a commercial Saccharomyces cerevisiae type was subjected to mutagenesis using ethyl methane sulfonate (EMS). Hence Ethanol tolerance investigation was carried out to isolate yeast strains from their natural habitats and to screen them for ethanol tolerance and ethanol production. Saccharomyces spp. was subjected to EMS (Ethylmethanesulphate) mutation of different concentration i.e., 0.1M, 0.2M, 0.3M, 0.4M in which 0.1M and 0.2M EMS (Ethyl methane sulphate) showed good growth of ethanol tolerance. Saccharomyces spp. were screened for their ethanol tolerance and showed good growth in medium containing 6-14 per cent ethanol. YPA and YSP isolates showed higher tolerance to ethanol stress and YOR with low tolerance while other isolates showed decreased growth under high ethanol concentration compared to original isolates. SSR profiling reflected polymorphism among Saccharomyces spp. However there was correlation between their genetic makeup and ethanol tolerance.
- Subjects
SACCHAROMYCES cerevisiae; RENEWABLE energy sources; BIOMASS energy; SACCHAROMYCETACEAE; LIGNOCELLULOSE
- Publication
Mysore Journal of Agricultural Sciences, 2019, Vol 53, Issue 1, p9
- ISSN
0047-8539
- Publication type
Article