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- Title
Enzyme degradation of protein in tobacco stem pulp.
- Authors
LIU Jing; XU Guang-jin; XIANG Hai-ying; WANG jian; WANG Bao-xing; CHEN Ling-feng; KONG Ning-chuan
- Abstract
[Objective]The optimal conditions of enzyme degradation were discussed to provide references for controlling protein in paper-process reconstituted tobacco. [Method]With Honghuadajinyuan as test material, enzyme degradation conditions including pH, temperature , slurry concentration, particle size, enzyme dosage and reaction time were investigated. [Result]The results showed that the appropriate enzymolysis conditions were as follows: pH of 6.4-7.2, reaction temperature of 35-55 °C, pulp concentration ⩽ 2%, particle size ⩾20 mesh, protease dosage of 400- 800 U/g and reaction time of 2 - 3 h. The rate of protein degradation in tobacco stem pulp could be about 55.70% under these conditions. [Conclusion] Under proper conditions , the protein in tobacco stem pulp should be effectively controlled with neutral protease.
- Publication
Journal of Southern Agriculture, 2014, Vol 45, Issue 11, p2036
- ISSN
2095-1191
- Publication type
Article
- DOI
10.3969/j:issn.2095-1191.2014.11.2036