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- Title
Expression of Melatonin and Glucocorticoid Receptor Varies along with Lung-Associated Cell-Mediated Immunity in the Jungle Bush Quail Perdicula asiatica: a Trade-off between Melatonin and Dexamethasone.
- Authors
Kharwar, Rajesh Kumar; Singh, Vaishali; Haldar, Chandana
- Abstract
The role of circulatory glucocorticoids along with melatonin in the regulation of lung-associated cell-mediated immunity has never been explored in any tropical bird. This could be interesting because glucocorticoids are immunosuppressive while melatonin is immunostimulatory in nature. In our present study, we report the localization and expression of the glucocorticoid and melatonin receptors and the effect of melatonin on dexamethasone-induced suppression of lung-associated and general immunity in the jungle bush quail Perdicula asiatica. The birds were treated with melatonin and dexamethasone at dose of 25µg/100g B.wt./day and 30µg/100g B.wt./day, respectively, for twenty eight days. At the end of the experiment, the birds were sacrificed and lung tissues and blood samples were collected for histology and morphometric analysis of lymphoid tissue (BALT and non-BALT nodules), total leukocyte count (TLC), lymphocyte count (LC), percent stimulation ratio of isolated lung lymphocytes (% SR), immunohistochemistry, western blot analysis and hormonal assay. Dexamethasone injection reduced the immune status in terms of the size of BALT and non-BALT nodules, TLC, LC and % SR, upregulated expression of the glucocorticoid receptor (GR) and downregulated expression of melatonin receptor types Mel1a and Mel1b. Melatonin administration increased the above immune parameters and upregulated expression of melatonin receptor types Mel1a and Mel1b while downregulating GR. Our results suggest the existence of the trade-off relationship between melatonin and corticosterone, which might be responsible for the regulation of lung-associated cell-mediated immunity under stress conditions via their receptors in the lung of the jungle bush quail Perdicula asiatica.
- Subjects
GLUCOCORTICOID receptors; CELLULAR immunity; MELATONIN; WESTERN immunoblotting; QUAILS; LOCUS coeruleus; LYMPHOCYTE count
- Publication
Journal of Evolutionary Biochemistry & Physiology, 2020, Vol 56, Issue 4, p364
- ISSN
0022-0930
- Publication type
Article
- DOI
10.1134/S0022093020040079