We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Bioinformatic Analysis of Circular RNA-Associated ceRNA Network Associated with Hepatocellular Carcinoma.
- Authors
Wu, Jiacheng; Liu, Shui; Xiang, Yien; Qu, Xianzhi; Xie, Yingjun; Zhang, Xuewen
- Abstract
Hepatocellular carcinoma (HCC) is the sixth most common cancer worldwide and is associated with a high mortality rate and poor treatment efficacy. In an attempt to investigate the mechanisms involved in the pathogenesis of HCC, bioinformatic analysis and validation by qRT-PCR were performed. Three circRNA GEO datasets and one miRNA GEO dataset were selected for this purpose. Upon combined biological prediction, a total of 11 differentially expressed circRNAs, 15 differentially expressed miRNAs, and 560 target genes were screened to construct a circRNA-related ceRNA network. GO analysis and KEGG pathway analysis were performed for the 560 target genes. To further screen key genes, a protein-protein interaction network of the target genes was constructed using STRING, and the genes and modules with higher degree were identified by MCODE and CytoHubba plugins of Cytoscape. Subsequently, a module was screened out and subjected to GO enrichment analysis and KEGG pathway analysis. This module included eight genes, which were further screened using TCGA. Finally, UBE2L3 was selected as a key gene and the hsa_circ_0009910–miR-1261–UBE2L3 regulatory axis was established. The relative expression of the regulatory axis members was confirmed by qRT-PCR in 30 pairs of samples, including HCC tissues and adjacent nontumor tissues. The results suggested that hsa_circ_0009910, which was upregulated in HCC tissues, participates in the pathogenesis of HCC by acting as a sponge of miR-1261 to regulate the expression of UBE2L3. Overall, this study provides support for the possible mechanisms of progression in HCC.
- Subjects
RNA analysis; GENE expression; GENES; HEPATOCELLULAR carcinoma; HUMAN genome; METABOLISM; POLYMERASE chain reaction; GENETIC testing; BIOINFORMATICS; ONTOLOGIES (Information retrieval); REVERSE transcriptase polymerase chain reaction; DISEASE progression; GENE expression profiling; MICRORNA
- Publication
BioMed Research International, 2019, p1
- ISSN
2314-6133
- Publication type
Article
- DOI
10.1155/2019/8308694