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- Title
A NOVEL PROTOCOL FOR TREATMENT AND ELIMINATION OF PASTEURELLA PNEUMOTROPICA INFECTION FROM RODENT COLONIES WITH SUBCLINICAL LATENT INFECTION.
- Authors
M., Harlev; M., Mujahed; T., Waner; R., Kalman
- Abstract
Pasteurella pneumotropica is a Gram-negative bacterium which poses a serious challenge to laboratory animal facilities. Elimination of the pathogen from rodent colonies is laborious and requires significant changes in regular standard operating procedures. In this study we attempted to develop a treatment protocol that would be both effective and compliant with standard work conditions for the elimination of chronic subclinical infection with P. pneumotropica. The aim of this study was to examine whether a treatment protocol for the elimination of chronic subclinical infection with Pp could be adapted to the standard operating procedures in a specified pathogen-free (SPF) rodent colony, without radically modifying the drinking water or diet, and without disrupting the normal replacement schedules of cages and water bottles. Enrofloxacin was added to acidified water once weekly at a Concentration of 0.0125%. A comparison of the mean inhibitory results between the enrofloxacin in acidified water and autoclaved water showed its effectiveness for at least 14 days. In this study acidified water/ enrofloxacin was replaced every week for a period of two weeks. Five groups of C57BL/6JOlaHsd mice were assigned to the experiment: Group1 served as the negative control. Group 2 and 3 served as the positive controls, and groups 4, 5 and 6. were the treatment groups. Positive control and treatment groups were infected with P. pneumotropica, and after confirmation of infection were treated for 2 weeks with enrofloxacin in the drinking water. The mice were monitored for up to 6 weeks post-treatment. Control groups were culture positive throughout the study whereas the negative control and treated groups were culture negative.
- Subjects
PASTEURELLA; ANIMAL experimentation; VETERINARY therapeutics; PASTEURELLOSIS; FLUOROQUINOLONES; RODENTS as carriers of disease
- Publication
Israel Journal of Veterinary Medicine, 2009, Vol 64, Issue 4, p108
- ISSN
0334-9152
- Publication type
Article