A simple and inexpensive sample preparation method followed by reversed-phase high performance liquid chromatography (RP-H PLC) was developed to quantify oxytetracycline (OTC), chlortetraCyClifle (CTC), and tetracycline (TC) residues in prawns. The total analytical time, including sample preparation, was 15 mm for each sample and no organic solvents were used in the sample preparation or chromatography stages.