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- Title
Sensitive, Rapid and Precise Determination of L-Glutamic Acid in Cheese using a Flow-Injection System with Immobilized Enzyme Column.
- Authors
PUCHADES, R.; LEMIEUX, L.; SIMARD, R. E.
- Abstract
Glutamic acid in cheese was determined by its reaction in a packed-bed enzyme reactor containing immobilized glutamate dehydrogenase (GIDH) in a flow-injection system, measuring fluorescence of NADH generated. There was a linear relationship (r = 0.999) between log-peak height and log-glumatic acid concentration (0.01 to 0.5 mM). The detection limit was 0.005 mM for an injection volume of 88 μL. The sample frequency was 30/h and the precision was better than 1.2% for 10 successive assays. The accuracy of the enzyme reactor-flow injection system was evaluated by comparison with HPLC results and an excellent correlation was obtained (r = 0.996). No measurable decrease in activity of the GIDH column was observed after a 3-month period.
- Subjects
GLUTAMIC acid; GLUTAMATE dehydrogenase; FLUORESCENCE; CHEESE; FLOW injection analysis
- Publication
Journal of Food Science (Wiley-Blackwell), 1989, Vol 54, Issue 2, p423
- ISSN
0022-1147
- Publication type
Article
- DOI
10.1111/j.1365-2621.1989.tb03098.x