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- Title
Rutenyum Mediyatör Kompleksi Temelli Galaktoz Biyosensörü Geliştirilmesi.
- Authors
CANBAY, Erhan; KUNDAKÇI, Sevval; YILDIZ, Ezginur; ÇELİK CANBAY, Zeynep; DOĞAN, Serçin; AKYILMAZ, Erol
- Abstract
Measurement of galactose level is of clinical significance in terms of galactosemia and galactose intolerance. Especially in newborns, it can have fatal consequences if it is not detected immediately. After diagnosis, galactose should be removed from the diet. Therefore, the determination of galactose in both blood and diet, especially milk and dairy products, is of great importance. In the literature data, the methods developed for galactose determination are time consuming and relatively slow methods that require pre-processing and expensive equipment such as spectrophotometer, chromatography, mass spectrometer. The aim of this study is to develop a fast, specific, inexpensive electrochemical biosensor system for galactose determination.In this study, in which Galactose Oxidase (GaOX) enzyme was used as a biocomponent, a carbon paste electrode containing a ruthenium mediator complex was used as the working electrode. GaOX enzyme was immobilized in the paste structure. The originality of the study is the use of the Ruthenium mediator system used in the paste structure. Electrode construction stages were characterized by cyclic voltammetry (CV) method, while optimization and characterization studies were carried out by means of DP between 0 & 0.9 V and amperometric measurements at 0.5 V. The studies also used a 1 mM p-benzoquinone mediator system in working buffer. As a result of optimization studies, optimum pH was found to be 6.5 and optimum temperature was 30 oC. While the linear detection range of the developed biosensor was found between 0.05- mM, the repeatability was found to be 0.24 mM on average, the standard deviation ± 0.021 and the coefficient of variation (CV%) as 8.3% for 0.25 mM galactose. No interference was observed in the interference effect trial. Sample analysis was made on lyophilized serum and lactose-free milk samples and % recovery was calculated. The use of the Ruthenium mediator complex used in this project in the production of biosensors has been rarely encountered in the literature. However, with this study, it was used for the first time in a galactose biosensor and for the first time with a carbon paste electrode.
- Subjects
GALACTOSEMIA; GALACTOSE; BIOSENSORS
- Publication
Journal of the Institute of Science & Technology / Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 2023, Vol 13, Issue 4, p2769
- ISSN
2146-0574
- Publication type
Article
- DOI
10.21597/jist.1321228