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- Title
In vitro Propagation of Codonopsis pilosula "Fengdang".
- Authors
Jianyun ZHOU; Wenbing ZHAO; Jian ZHOU
- Abstract
[Objectives] The paper was to establish in vitro propagation of an excellent germplasm of Codonopsis pilosula "Fengdang". [Methods] The effects of different plant growth regulators on callus induction and redifferentiation were tested by using sterile stem expiants in vitro that germinated from seeds of superior "Fengdang" plant. [Results] The optimum medium for callus induction was MS +0.5 mg/L NAÂ + 1.0 mg/L 16-BA. The optimal medium for bud differentiation was MS + 0.1 mg/L NAA +0.5 mg/L 6-BA, and that for rooting was 1/2 MS + 0.2 mg/L NAA +0. 2 mg/L 1BA. By using the media mentioned above, the rates of callus induction, bud differentiation and rooting reached 91 %, 100% and 97%, respectively. [Conclusions] The rapid propagation system of regenerated plants in vitro established in this study lays a foundation for the popularization and industrialized seedling of excellent germplasm of "Fengdang".
- Subjects
PLANT regulators; REGENERATION (Botany); CALLUS
- Publication
Medicinal Plant, 2022, Vol 13, Issue 6, p27
- ISSN
2152-3924
- Publication type
Article
- DOI
10.19600/j.cnki.issn2152-3924.2022.06.006