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- Title
Decoding Gene Networks Modules That Explain the Recovery of Hymenoglossum cruentum Cav. After Extreme Desiccation.
- Authors
Ostria-Gallardo, Enrique; Larama, Giovanni; Berríos, Graciela; Fallard, Ana; Gutiérrez-Moraga, Ana; Ensminger, Ingo; Manque, Patricio; Bascuñán-Godoy, Luisa; Bravo, León A.
- Abstract
Hymenoglossum cruentum (Hymenophyllaceae) is a poikilohydric, homoiochlorophyllous desiccation-tolerant (DT) epiphyte fern. It can undergo fast and frequent dehydration-rehydration cycles. This fern is highly abundant at high-humidity/low-light microenvironments within the canopy, although rapid changes in humidity and light intensity are frequent. The objective of this research is to identify genes associated to desiccation-rehydration cycle in the transcriptome of H. cruentum to better understand the genetic dynamics behind its desiccation tolerance mechanism. H. cruentum plants were subjected to a 7 days long desiccation-rehydration process and then used to identify key expressed genes associated to its capacity to dehydrate and rehydrate. The relative water content (RWC) and maximum quantum efficiency (F v/ F m) of H. cruentum fronds decayed to 6% and 0.04, respectively, at the end of the desiccation stage. After re-watering, the fern showed a rapid recovery of RWC and F v/ F m (ca. 73% and 0.8, respectively). Based on clustering and network analysis, our results reveal key genes, such as UBA/TS-N , DYNLL , and LHC , orchestrating intracellular motility and photosynthetic metabolism; strong balance between avoiding cell death and defense (CAT3 , AP2/ERF) when dehydrated, and detoxifying pathways and stabilization of photosystems (GST , CAB2 , and ELIP9) during rehydration. Here we provide novel insights into the genetic dynamics behind the desiccation tolerance mechanism of H. cruentum.
- Subjects
QUANTUM efficiency; PLANT defenses; LIGHT intensity; CELL motility; TEMPERATE rain forests; CELL death; GENE regulatory networks
- Publication
Frontiers in Plant Science, 2020, Vol 11, p1
- ISSN
1664-462X
- Publication type
Article
- DOI
10.3389/fpls.2020.00574