We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Acer mono Extract Inhibits Invasive Activities and G1/S Transition of HT1080 Fibrosarcoma Cells.
- Authors
Jin Hee Kim; Gwang Ha Hwang; Hyun Jung Kim; Songhee Jeon; Boo Ahn Shin
- Abstract
Acer mono is known to contain bioactive substances that exhibit beneficial effects in osteoporosis, gastric ulcers, hepatic damage, and pathologic angiogenesis. The current study aimed to investigate the effects of Acer mono extract on the invasive activities and cell-cycle progression of human fibrosarcoma cells. Cytotoxicity of Acer mono extract was assessed by MTT assay, in-vitro invasiveness of HT1080 fibrosarcoma cells was measured using matrigel assay, expression of invasion- and cell-cycle-related proteins was analyzed by western blot analysis, and that of E2F target genes was quantified using qRT-PCR. Acer mono extract did not show distinct cytotoxicity in the experimental concentrations used. Invasiveness of HT1080 fibrosarcoma cells and expression of cyclin D1 and CDK4 in them were significantly reduced in a dose-dependent manner after treatment with Acer mono extract. Acer mono extract showed inhibitory effects on the G1/S transition during cell-cycle progression; the active phosphorylated Rb protein level was decreased, and expression of E2F target genes was downregulated by the Acer mono extract. Our data collectively demonstrated that Acer mono extract exerts inhibitory effects on the invasiveness and cell-cycle progression of HT1080 human fibrosarcoma cells.
- Subjects
CELL cycle proteins; MAPLE; FIBROSARCOMA; STOMACH ulcers; WESTERN immunoblotting; CYCLIN-dependent kinases
- Publication
Chonnam Medical Journal, 2021, Vol 57, Issue 3, p185
- ISSN
2233-7385
- Publication type
Article
- DOI
10.4068/cmj.2021.57.3.185