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- Title
CD41 is a reliable identification and activation marker for murine basophils in the steady state and during helminth and malarial infections.
- Authors
Bakocevic, Nadja; Claser, Carla; Yoshikawa, Soichiro; Jones, Leigh Ann; Chew, Samantha; Goh, Chi Ching; Malleret, Benoit; Larbi, Anis; Ginhoux, Florent; Lafaille, Maria Curotto; Karasuyama, Hajime; Renia, Laurent; Ng, Lai Guan
- Abstract
Basophils, a rare leukocyte population in peripheral circulation, are conventionally identified as CD45int CD49b+Fcε RI+ cells. Here, we show that basophils from blood and several organs of naïve wild-type mice express CD41, the α subunit of αIIbβ3 integrin. CD41 expression on basophils is upregulated after in vivo IL-3 treatment and during infection with Nippostrongylus brasiliensis ( Nb). Moreover, CD41 can be used as a reliable marker for basophils, circumventing technical difficulties associated with Fcε RI for basophil identification in a Nb infection model. In vitro anti- Ig E cross-linking and IL-3 basophil stimulation showed that CD41 upregulation positively correlates with augmented surface expression of CD200 R and increased production of IL-4/ IL-13, indicating that CD41 is a basophil activation marker. Furthermore, we found that infection with Plasmodium yoelii 17X ( Py17x) induced a profound basophilia and using Mcpt8DTR reporter mice as a basophil-specific depletion model, we verified that CD41 can be used as a marker to track basophils in the steady state and during infection. During malarial infection, CD41 expression on basophils is negatively regulated by IFN-γ and positively correlates with increased basophil IL-4 production. In conclusion, we provide evidence that CD41 can be used as both an identification and activation marker for basophils during homeostasis and immune challenge.
- Publication
European Journal of Immunology, 2014, Vol 44, Issue 6, p1823
- ISSN
0014-2980
- Publication type
Article
- DOI
10.1002/eji.201344254