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- Title
Cytotoxic Effect of Vitexin Compoundon Aß - Induced BV2 Cells.
- Authors
Yahaya, Muhamad Afiq Faisal; Murizal, Zainol; Nordin, Norshariza; Stanslas, Johnson; Zulfadli, Mehat Muhammad
- Abstract
Neuroinflammation is a series of neuropathological processes as the result from the interaction with the microenvironmental cues (e.g. lipopolysaccharide (LPS) and amyloid-ß (Aß)) present at the central nervous system (CNS). The neuroinflammation will become chronic when the inflammation persists which lead to the neuronal damage and eventually neurodegeneration development. Thus, the present study aimed to determine the cytotoxic effect of vitexin compound on BV2 cells and Aß-induced BV2 cells. Cell viability assay was conducted to investigate the toxicity effect of the compound towards both cell cultures. Six concentrations of vitexin were prepared and tested. MTT assay (3-(4, 5-Dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide) was performed to determine the cytotoxicity of the compound. The results from both cultures were expressed as Means ± S.D where p<0.05 is considered as significant. The result from this study showed that the vitexin compound possesses cytotoxicity effect in dose-dependent manner upon exposed to both cultures. The IC50 from both cell cultures was 50µM. The result suggests that the vitexin compound is safe to be used in both conditions. This preliminary finding allows the future research to be conducted on vitexin in search for the potential treatment for neurodegenerative diseases.
- Subjects
CENTRAL nervous system; INFLAMMATION; CELL culture; CELLS; NEURODEGENERATION
- Publication
Current Trends in Biotechnology & Pharmacy, 2020, p109
- ISSN
0973-8916
- Publication type
Article