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- Title
Molecular characterization of Eae and Stxs genes for E. coli O157:H7 isolates from Calves.
- Authors
Suleiman, Jassim Mohamed; Saed, Omar-Althani Shareef; AL-Kubaisi, S. M. A.; Hasan, Mustafa Salah; Owain, Maher Saber; Hussein, Mohammed Ali; Jarad, Ahmed Sami
- Abstract
This study aimed to detect eae and Stx genes of thirty two E. coli O157:H7 isolates recovered from calves. These isolates were isolated and identify by traditional methods of culturing and latex agglutination test from fecal swabs of four diarrheic calves and twenty eight apparently healthy calves. The eae and Stx genes were detected by a realtime PCR applied on the extracted bacterial DNA. The results showed that 19 isolates had stx1 gene, 10 isolates had stx2 gene and 17 isolates had eae gene. It is concluded that molecular characterization by using real time PCR is a good test for confirming infection with E. coli O157:H7 and detection of their eae and Stx genes .
- Subjects
DIAGNOSIS of escherichia coli diseases; AGGLUTINATION tests; VETERINARY medicine; CATTLE; DIARRHEA; DNA; ANIMAL experimentation; MOLECULAR pathology; GENES; MICROBIAL virulence; POLYMERASE chain reaction
- Publication
Medico-Legal Update, 2020, Vol 20, Issue 1, p788
- ISSN
0971-720X
- Publication type
Article
- DOI
10.37506/v20/i1/2020/mlu/194421