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- Title
Efficient plant regeneration mediated by root segment-derived juvenile shoots of Rhus chinensis Mill.
- Authors
Hou, Jinyan; Ding, Shuangshuang; Su, Pengfei; Wang, Dacheng; Wu, Lifang
- Abstract
Key message: An efficient micropropagation procedure for Rhus chinensis was developed using root segment-derived juvenile shoots as the source of materials through multiple shoot induction and hydroponic acclimation. Rhus chinensis Mill. is an economically important multipurpose tree in the Anacardiaceae that has a wide range of medicinal, ornamental, and industrial values. Here, an efficient micropropagation procedure for R. chinensis was developed using leaves, nodal segments, and petioles of juvenile shoots obtained from root segments as explants. The highest shoot induction frequency (100%) with a mean number of shoots (20.0 ± 0.9) was obtained when in vitro nodal segments were cultured on MS medium supplemented with 0.2 mg l−1 TDZ. The shoot clusters were transferred to medium containing 0.2 mg l−1 TDZ and 0.1 mg l−1 NAA for multiplication, and shoot elongation was observed on medium with 0.2 mg l−1 6-BA and 0.2 mg l−1 GA3. The highest rooting frequency (91.1%) was achieved on 1/4 MS medium fortified with 2.0 mg l−1 NAA. The major challenge of the low transplantation survival rate of regenerated plants was solved by adopting an ex vitro hydroponic acclimation system. This is the first report on the propagation of R. chinensis through direct shoot organogenesis and hydroponic acclimation. The present procedure provides a novel method for the large-scale propagation and germplasm conservation of R. chinensis.
- Subjects
REGENERATION (Botany); GERMPLASM conservation; GERMPLASM; MULTIPURPOSE trees; ACCLIMATIZATION; ANACARDIACEAE; RIFLE-ranges
- Publication
Trees: Structure & Function, 2023, Vol 37, Issue 4, p1055
- ISSN
0931-1890
- Publication type
Article
- DOI
10.1007/s00468-023-02405-5