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- Title
'Nonspecific' Blocking of Human Ovarian Carcinoma-Associated Cellular Cytotoxicity In Vitro.
- Authors
Saksela, E.; Penttinen, K.; Pyrhönen, S.
- Abstract
The blocking ability of heat-aggregated human polyclonal IgG and complexes formed by preservative-free tetanus toxoid (TT) and human anti-tetanus serum was investigated on the cell-mediated tumor-specific cytotoxicity reaction of ovarian carcinoma patients against cultured ovarian carcinoma cells. Aggregated IgG effectively blocked the cytotoxic reaction when the purified effector cells were preincubated with various concentrations of this material, whereas no blocking activity was detected when target cells were preincubated and washed before addition of cytotoxic lymphocytes. Optimal concentration was about 1.6 μg/ml, and higher or lower concentrations were less effective. Anti-TT/TT immunocomplexes behaved similarly and could effectively block at the effector cell but not at the target cell level. The results suggest that the effector cells in human ovarian carcinoma-associated cellular cytotoxicity are Fc-receptor-carrying cells and that their cytotoxic activity can be 'nonspecifically' blocked by saturating these sites with aggregated IgG or unrelated immunocomplexes.
- Subjects
IMMUNOGLOBULIN G; CANCER; CLOSTRIDIUM diseases; BLOOD plasma; LEUCOCYTES; SERUM
- Publication
Scandinavian Journal of Immunology, 1974, Vol 3, Issue 6, p781
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1974.tb01313.x