We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Characterization of Leishmania (L.) infantum chagasi in visceral leishmaniasis associated with hiv co-infection in Northeastern Brazil.
- Authors
de Lima, Elaine Cristina Bomfim; Barbosa Júnior, Walter Lins; de Brito, Maria Edileuza Felinto; de Melo, Fábio Lopes; Brandão Filho, Sinval Pinto; de Medeiros, Zulma Maria
- Abstract
Visceral leishmaniasis, associated with HIV/AIDS coinfection, is becoming a more aggressive disease, complicating an accurate prognosis. A 21-year-old HIV-positive female presenting with clinical features of visceral leishmaniasis was enrolled in this study. Bone marrow cytology, Novy-MacNeal-Nicolle culture and kDNA PCR of peripheral blood were all positive. Typing methods, multilocus enzyme electrophoresis and ITS1-RFLP PCR of peripheral blood confirmed infection by Leishmania (L.) infantum chagasi. PCR has proved to be safer and more affordable than other characterization methods; ITS1-RFLP PCR can diagnose and type Leishmania spp. in both endemic and non-endemic areas, favoring the prognosis and allowing the appropriate treatment of patients.
- Subjects
VISCERAL leishmaniasis; LEISHMANIASIS; LEISHMANIA donovani; LEISHMANIA mexicana; PATHOGENIC microorganisms
- Publication
Revista do Instituto de Medicina Tropical de São Paulo, 2017, Vol 59, p1
- ISSN
0036-4665
- Publication type
Article
- DOI
10.1590/S1678-9946201759048