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- Title
Canscora lucidissima, a Chinese folk medicine, exerts anti-inflammatory activities by inhibiting the phosphorylation of ERK1/2 in LPS-activated macrophages.
- Authors
Fei, Qiao-ling; Zhang, Xiao-yu; Qi, Rui-juan; Huang, Yun-feng; Han, Yi-xin; Li, Xi-meng; Cai, Run-lan; Gao, Yuan; Qi, Yun
- Abstract
Background: Canscora lucidissima (Levl. & Vaniot) Hand.-Mazz. (C. lucidissima), mainly distributed in southern China, has been shown to be effective in the treatment of inflammatory diseases. However, the underlying mechanism of its anti-inflammatory effect is not fully understood. Methods: In this study, we investigated the anti-inflammatory mechanism of ethanol extract of C. lucidissima (Cl-EE) in lipopolysaccharide (LPS)-induced inflammatory models. ELISA, real-time PCR, Western blot and luciferase reporter assay were used for the experiments in vitro, and ICR mouse endotoxemia model was used for in vivo test. Results: Our data showed that Cl-EE reduced the production of NO by down-regulating the mRNA and protein expression of inducible nitric oxide synthase (iNOS) in LPS-activated RAW 264.7 cells. Meanwhile, it potently decreased other proinflammatory mediators, such as TNF-α, IL-6, MCP-1 and IL-1β at the transcriptional and translational levels. Further study indicated that Cl-EE did not affect NF-κB signaling pathway but significantly suppressed the phosphorylation of ERK1/2, rather than JNK or p38. In a LPS-induced endotoxemia mouse model, a single intraperitoneal injection of Cl-EE (75–300 mg/kg) could lower circulatory TNF-α, IL-6 and MCP-1 levels. Conclusions: Collectively, our results indicated that Cl-EE suppressed the phosphorylation level of ERK1/2 thus reducing the transcription and translation of inflammatory genes, thereby exerted anti-inflammatory activity. This study reveals the anti-inflammatory mechanism of C. lucidissima and may provide an effective treatment option for a variety of inflammatory diseases.
- Subjects
ENDOTOXEMIA prevention; ANIMAL experimentation; BIOLOGICAL assay; CELL lines; CHEMOKINES; ENZYME-linked immunosorbent assay; ETHANOL; GENE expression; HERBAL medicine; INFLAMMATORY mediators; INTRAPERITONEAL injections; INTERLEUKIN-1; INTERLEUKINS; MACROPHAGES; CHINESE medicine; MESSENGER RNA; MICE; NITRIC acid; PHOSPHORYLATION; POLYMERASE chain reaction; PROTEIN kinases; TUMOR necrosis factors; WESTERN immunoblotting; NITRIC-oxide synthases; ENDOTOXEMIA; LIPOPOLYSACCHARIDES; IN vitro studies; IN vivo studies; DRUG administration; DRUG dosage; PHARMACODYNAMICS
- Publication
BMC Complementary & Alternative Medicine, 2019, Vol 19, Issue 1, p1
- ISSN
1472-6882
- Publication type
Article
- DOI
10.1186/s12906-019-2783-2