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- Title
Green tea supplementation alters gene transcripts involved in hepatic fat oxidation and synthesis in rats fed high fat diets.
- Authors
Bezzina, R.; Chen, N.; Mathai, M. L.; Carey, K. A.; Cameron-Smith, D.; Weisinger, H. S.; Sinclair, A. J.; Weisinger, R. S.; Lewandowski, P.
- Abstract
Background -- Green Tea has been shown to induce many health related benefits including reductions in bodyweight and waist circumference, decreased insulin resistance and improvement in blood lipid profiles all of which are implicated in the aetiology of non-alcoholic fatty liver disease. However, to date little is known about the effect of green tea on the molecular mechanisms regulating hepatic lipid synthesis, storage and oxidation. Objectives -- This study investigated the impact of green tea, tea catechins or black tea supplementation on rodent liver gene transcripts essential for lipid oxidation and synthesis, and on liver fat accumulation. Design -- Sprague-Dawley rats were fed a high fat diet and supplemented at 100% of their fluid intake with water as a control (n=7), green tea (n=7), epigallocatechin-3-gallate (EGCG) (n=7) or black tea (n=7) from 4 weeks age for a period of 6 months. Following supplementation the mRNA levels of Peroxisome Proliferator Receptor Alpha (PPARα), Carnitine Palmitoyl Transferase 1 (CPT1), Acetyl CoA Oxidase (ACO), Sterol Regulatory Element Binding Protein 1c (SREBP-1c), Fatty Acid Synthase (Fsynth), Malonyl CoA Decarboxylase (MCD) and Acetyl CoA Carboxylase (ACC) were measured. Histology and triglyceride analysis was performed to establish the extent of fatty infiltration in the liver. Outcomes -- Green tea and black tea supplementation significantly decreased the rodents fat mass and increased the expression of all genes examined, particularly ACO (GT = 20 fold, BT = 14 fold) and MCD (GT = 12 fold, BT = 13 fold). The supplementation with green and black tea also resulted in greater fatty infiltration of the liver than both the control and the EGCG groups. Conclusions -- Green and black tea, but not EGCG, appear to cause an increase in the amount of fatty infiltration in the liver, possibly as a consequence of the increased expression of lipid synthesis genes. The concomitant increase in the expression of genes involved in the metabolism of fat suggests that fatty acid turnover in the liver was increased.
- Subjects
GREEN tea; GENETIC transcription; DIET; RATS; TEA
- Publication
Asia Pacific Journal of Clinical Nutrition, 2005, Vol 14, pS85
- ISSN
0964-7058
- Publication type
Article