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- Title
Characterization of the Bm61 of the Bombyx mori Nucleopolyhedrovirus.
- Authors
Hongxing Shen; Keping Chen; Qin Yao; Yang Zhou
- Abstract
orf61 ( bm61) of Bombyx mori Nucleopolyhedrovirus (BmNPV) is a highly conserved baculovirus gene, suggesting that it performs an important role in the virus life cycle whose function is unknown. In this study, we describe the characterization of bm61. Quantitative polymerase chain reaction (qPCR) and western blot analysis demonstrated that bm61 was expressed as a late gene. Immunofluorescence analysis by confocal microscopy showed that BM61 protein was localized on nuclear membrane and in intranuclear ring zone of infected cells. Structure localization of the BM61 in BV and ODV by western analysis demonstrated that BM61 was the protein of both BV and ODV. In addition, our data indicated that BM61 was a late structure protein localized in nucleus.
- Subjects
SILKWORMS; NUCLEOPOLYHEDROVIRUSES; BACULOVIRUS genetics; DNA polymerases; NUCLEAR membranes; PROTEIN analysis; CONFOCAL microscopy; POLYMERASE chain reaction; IMMUNOFLUORESCENCE
- Publication
Current Microbiology, 2009, Vol 59, Issue 1, p65
- ISSN
0343-8651
- Publication type
Article
- DOI
10.1007/s00284-009-9399-6