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- Title
Modular <sup>31</sup>P wideband inversion transfer for integrative analysis of adenosine triphosphate metabolism, T<sub>1</sub> relaxation and molecular dynamics in skeletal muscle at 7T.
- Authors
Ren, Jimin; Sherry, A. Dean; Malloy, Craig R.
- Abstract
Purpose: For efficient and integrative analysis of de novo adenosine triphosphate (ATP) synthesis, creatine‐kinase–mediated ATP synthesis, T1 relaxation time, and ATP molecular motion dynamics in human skeletal muscle at rest. Methods: Four inversion‐transfer modules differing in center inversion frequency were combined to generate amplified magnetization transfer (MT) effects in targeted MT pathways, including Pi ↔ γ‐ATP, PCr ↔ γ‐ATP, and 31Pγ(α)ATP ↔ 31PβATP. MT effects from both forward and reverse exchange kinetic pathways were acquired to reduce potential bias and confounding factors in integrated data analysis. Results: Kinetic data collected using 4 wideband inversion modules (8 minutes each) yielded the forward exchange rate constants, kPCr→γATP = 0.31 ± 0.05 s–1 and kPi→γATP = 0.064 ± 0.012 s–1, and the reverse exchange rate constants, kγATP→Pi = 0.034 ± 0.006 s–1 and kγATP→PCr = 1.37 ± 0.22 s–1, respectively. The cross‐relaxation rate constant, σγ(α) ↔ βATP was –0.20 ± 0.03 s–1, corresponding to ATP rotational correlation time τc of 0.8 ± 0.1 × 10–7 seconds. The intrinsic T1 relaxation times were Pi (9.2 ± 1.4 seconds), PCr (6.2 ± 0.4 seconds), γ‐ATP (1.8 ± 0.1 seconds), α‐ATP (1.4 ± 0.1 seconds), and β‐ATP (1.1 ± 0.1 seconds). Muscle ATP T1 values were found to be significantly longer than those previously measured in the brain using a similar method. Conclusion: A combination of multiple inversion transfer modules provides a comprehensive and integrated analysis of ATP metabolism and molecular motion dynamics. This relatively fast technique could be potentially useful for studying metabolic disorders in skeletal muscle.
- Publication
Magnetic Resonance in Medicine, 2019, Vol 81, Issue 6, p3440
- ISSN
0740-3194
- Publication type
Article
- DOI
10.1002/mrm.27686