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- Title
改良椎板切除法构建脊髓损伤模型大鼠.
- Authors
杜凯然; 邓 强; 郭铁峰; 张彦军; 彭冉东; 李军杰; 王雨榕; 张凯东; 罗林钊
- Abstract
BACKGROUND: Spinal cord injury has become a worldwide problem. Experimental animal modeling is the first step to explore the disease; however, there is a lack of experimental animal models that are more effective. OBJECTIVE: To establish a rat model of spinal cord injury that can be replicated, regulated, has little trauma, low mortality, low blood loss, wide application and short intraoperative time. METHODS: Forty Sprague-Dawley rats with similar body mass were selected and randomly divided into conventional model group and improved model group (n=20 per group), where the model of spinal cord injury was made using Allen’s method and improved method, respectively. In the improved model group, a dental drill was used for biting. Operation time, intraoperative blood loss, mortality, and Basso-Beattie-Bresnahan locomotor rating scale scores at 1, 3, 6, 9, 12, and 15 days after beating were compared between the two groups. RESULTS AND CONCLUSION: The improved method for constructing the animal model of secondary spinal cord injury has the advantages of shorter modeling time, less intraoperative blood loss, lower mortablity, and more centralized Basso-Beattie-Bresnahan score data as compared with the conventional method, which proves that the improved method is more suitable for establishing the model of secondary spinal cord injury.
- Subjects
SPINAL cord injuries; SURGICAL blood loss; DENTAL drilling; LABORATORY animals; SPRAGUE Dawley rats
- Publication
Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu, 2023, Vol 27, Issue 32, p5173
- ISSN
2095-4344
- Publication type
Article
- DOI
10.12307/2023.825