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- Title
Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia.
- Authors
Kristiana, Rhesi; Bedoux, Gilles; Pals, Gerard; Wayan Mudiantaa, I.; Taupin, Laure; Marty, Christel; Asagabaldan, Meezan Ardhanu; Ayuningrum, Diah; Trianto, Agus; Bourgougnon, Nathalie; Radjasa, Ocky Karna; Sabdono, Agus; Hanafi, Muhammad
- Abstract
The aims of this work are to isolate bacterial symbionts from nudibranchs and subsequently to determine anti-Methicillin resistant Staphylococcus aureus (MRSA), cytotoxicity and anti-Herpes simplex virus type 1 (HSV-1) activities of bio compounds. A total of 15 species of nudibranchs were collected from Karimunjawa and five species from Bali, respectively. A total of 245 bacteria isolates were obtained. The anti-MRSA activity screening activity indicated two active bacteria. Ethyl acetate extracts from supernatants, indicating extracelullar compounds, showed an inhibition zone against MRSA at concentrations of 500-1,000 μg/ml. DNA sequence analysis showed that the strain KJB-07 from Phyllidia coelestis was closely related to Pseudoalteromonas rubra, whereas the strain NP31-01 isolated from Phyllidia varicosa was closely related to Virgibacillus salarius. The extract of Pseudoalteromonas rubra was cytotoxic to Vero cells at a concentration of 75 μg/ml. The extract of V. salarius presented no cytotoxicity at concentrations of 5-1,000 μg/ml. No anti HSV-1 was observed for both isolated bacteria. This is the first study describing research on anti-MRSA, cytotoxicity and anti HSV-1 activity of bacterial symbionts from the viscera of nudibranch. Compounds produced by Pseudoalteromonas rubra and V. salarius, had potential anti-MRSA activity. However, only extracts from Pseudoalteromonas rubra showed cytotoxic effects on Vero cells. Three compounds were identified by LC/MS after purification from culture supernatant.
- Subjects
INDONESIA; NUDIBRANCHIA; BACTERIA; DNA analysis; NUCLEOTIDE sequence; STAPHYLOCOCCUS aureus; ETHYL acetate; HUMAN herpesvirus 1
- Publication
PeerJ, 2020, p1
- ISSN
2167-8359
- Publication type
Article
- DOI
10.7717/peerj.8093