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- Title
Enzymatic Bioelectrocatalysis for Enzymology Applications.
- Authors
Kummer, Matthew J.; Minteer, Shelley D.
- Abstract
Enzymatic biosensors are commonly used to evaluate the concentration of individual analytes in samples (e. g., glucose in blood, lactate in sweat, etc.), where the working electrode utilizes an enzyme (a "bioelectrode") as the chemically selective agent for electrochemical analyses. However, those same enzymatic bioelectrodes can be used for the analytical characterization of enzymes as a new biophysical characterization tool for enzymologists. This Minireview will detail recent work focused on utilizing enzymatic bioelectrocataysis for enzymology applications. Specifically, it will talk about the use of enzymatic bioelectrodes for studying kinetics, inhibition, transport, and thermodynamics. It will also compare and contrast electrochemical techniques with spectrophotometric techniques for enzymology.
- Subjects
ENZYMOLOGY; BLOOD sugar; GLUCOSE analysis; ELECTROCHEMICAL analysis; THERMODYNAMICS; BIOSENSORS
- Publication
ChemElectroChem, 2020, Vol 7, Issue 10, p2222
- ISSN
2196-0216
- Publication type
Article
- DOI
10.1002/celc.202000239