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- Title
Epigallocatechin Gallate Promotes the Development of Mouse 2-Cell Embryos In Vitro by Regulating Mitochondrial Activity and Expression of Genes Related to p53 Signalling Pathway.
- Authors
Zhang, Weiyu; Lv, Junjie; Zhang, Yanqin; Jiang, Yufei; Chu, Chenfeng; Wang, Shie
- Abstract
Preliminary studies have found that the epigallocatechin gallate (EGCG) at proper concentration could promote development of pre-implantation mouse embryos in vitro. However, the underlying mechanisms have not been well understood. In this study, we collected 1-cell embryos from Kunming (KM) mice, cultured them in M16 medium or M16 medium supplemented with 10 μg/mL EGCG and investigated the effects of EGCG on mitochondrial activity and reactive oxygen species (ROS) level of 2-cell embryos. Furthermore, we explored expression differences of genes related to p53 signalling pathway in 2-cell embryos using a PCR array. The results showed that ROS level and mitochondrial membrane potential were significantly lower in embryos cultured in the EGCG group than in the M16 group (p < 0.05), while the adenosine triphosphate content was slightly lower than in the M16 group (p > 0.05). PCR array test results showed that 18 genes were differentially expressed, among which eight genes involving cell growth, cell cycle regulation and mRNA transcription were up-regulated and 10 genes involving apoptosis, cell cycle arrest and DNA repair were down-regulated in the EGCG groups. It is concluded that EGCG could promote the development of l-cell embryos in vitro possibly due to its ability to scavenge ROS and regulate mitochondrial activity. In addition, EGCG could influence expression of genes related to p53 signalling pathway in 2-cell embryos and promote cell cycle progression.
- Subjects
EPIGALLOCATECHIN gallate; LABORATORY mice; MITOCHONDRIAL physiology; P53 antioncogene; GENETIC regulation; IN vitro studies
- Publication
Basic & Clinical Pharmacology & Toxicology, 2014, Vol 115, Issue 5, p403
- ISSN
1742-7835
- Publication type
Article
- DOI
10.1111/bcpt.12252