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- Title
Impact of the Peptide WMR-K on Dual-Species Biofilm Candida albicans/Klebsiella pneumoniae and on the Untargeted Metabolomic Profile.
- Authors
Galdiero, Emilia; Salvatore, Maria Michela; Maione, Angela; Carraturo, Federica; Galdiero, Stefania; Falanga, Annarita; Andolfi, Anna; Salvatore, Francesco; Guida, Marco
- Abstract
In recent years, the scientific community has focused on the development of new antibiotics to address the difficulties linked to biofilm-forming microorganisms and drug-resistant infections. In this respect, synthetic antimicrobial peptides (AMPs) are particularly regarded for their therapeutic potential against a broad spectrum of pathogens. In this work, the antimicrobial and antibiofilm activities of the peptide WMR-K towards single and dual species cultures of Candida albicans and Klebsiella pneumoniae were investigated. We found minimum inhibitory concentration (MIC) values for WMR-K of 10 µM for K. pneumoniae and of 200 µM for C. albicans. Furthermore, sub-MIC concentrations of peptide showed an in vitro inhibition of biofilm formation of mono and polymicrobial systems and also a good biofilm eradication even if higher concentrations of it are needed. In order to provide additional evidence for the effect of the examined peptide, a study of changes in extracellular metabolites excreted and/or uptaken from the culture medium (metabolomic footprinting) in the poly-microbial association of C. albicans and K. pneumoniae in presence and absence of WMR-K was performed. Comparing to the untreated dual species biofilm culture, the metabolomic profile of the WMR-K treated culture appears significantly altered. The differentially expressed compounds are mainly related to the primary metabolic pathways, including amino acids, trehalose, pyruvic acid, glycerol and vitamin B6.
- Subjects
KLEBSIELLA pneumoniae; CANDIDA albicans; METABOLOMICS; TREHALOSE; BIOFILMS; MICROBIAL metabolites; VITAMIN B6; PEPTIDE antibiotics
- Publication
Pathogens, 2021, Vol 10, Issue 2, p214
- ISSN
2076-0817
- Publication type
Article
- DOI
10.3390/pathogens10020214