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- Title
Ômega-conotoxina MVIIC no trauma experimental da medula espinhal em ratos.
- Authors
Sebastian Gutiérrez-Lozano, Juan; Paula Rajão, Maria; Osorio, Carla; Rubatino, Fernando; Marliére, Marina; Gonçalves de Melo, Eliane
- Abstract
Introduction: Spinal cord lesions produce an exacerbated calcium input, being the most critical step after the spinal cord injury, mainly due to the activation of voltage-dependent calcium channels. Thus, calcium channel blockers could have a high potential to reduce spinal cord injuries. Aim: To evaluate the neuroprotective effect of omega-conotoxin MVIIC obtained from the poison of Conus magus in rats with spinal cord trauma. Methods: Thirty-six adult, male, Wistar rats were randomly divided into six groups. Animals in the negative control group underwent dorsal laminectomy. In the other groups, in addition to laminectomy, the animals underwent acute bruised trauma using the MASCIS impactor. Intrathecal placebo application was performed in the animals of the positive control groups. In groups G3 and G5 doses of 15 and 30 pmol of the Omega-conotoxin toxin MVIIC were applied, respectively, in the treated animals, 5 minutes after the trauma. In groups G4 and G6, doses of 15 and 30 pmol were applied, respectively, one hour after the trauma. Segments of the spinal cord were collected to quantify reactive oxygen species and lipid peroxidation, and to assess gene expression of factors related to apoptosis using a qRT-PCR technique. Results: No significant differences were found for the treatments evaluated regarding the free radical production and reactions of lipid peroxidation. However, the use of 15 pmol of omega-conotoxin MVIIC 1 hour after trauma tended to be better than the other evaluated doses. Conclusions: Omegaconotoxin MVIIC could be useful for the treatment of spinal cord trauma in rats. However, further studies are necessary to determine the adequate dose of this substance.
- Subjects
FREE radical reactions; SPINAL cord; SPINAL cord injuries; CALCIUM channels; REACTIVE oxygen species; LIPID peroxidation (Biology); CALCIUM antagonists
- Publication
Veterinary & Animal Science / Veterinaria y Zootecnia, 2019, Vol 13, Issue 1, p99
- ISSN
2011-5415
- Publication type
Article
- DOI
10.17151/vetzo.2019.13.1.8