We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cold adaptation shapes the robustness of metabolic networks in Drosophila melanogaster.
- Authors
Williams, Caroline M.; Watanabe, Miki; Guarracino, Mario R.; Ferraro, Maria B.; Edison, Arthur S.; Morgan, Theodore J.; Boroujerdi, Arezue F. B.; Hahn, Daniel A.
- Abstract
When ectotherms are exposed to low temperatures, they enter a cold-induced coma (chill coma) that prevents resource acquisition, mating, oviposition, and escape from predation. There is substantial variation in time taken to recover from chill coma both within and among species, and this variation is correlated with habitat temperatures such that insects from cold environments recover more quickly. This suggests an adaptive response, but the mechanisms underlying variation in recovery times are unknown, making it difficult to decisively test adaptive hypotheses. We use replicated lines of Drosophila melanogaster selected in the laboratory for fast (hardy) or slow (susceptible) chill-coma recovery times to investigate modifications to metabolic profiles associated with cold adaptation. We measured metabolite concentrations of flies before, during, and after cold exposure using nuclear magnetic resonance (NMR) spectroscopy to test the hypotheses that hardy flies maintain metabolic homeostasis better during cold exposure and recovery, and that their metabolic networks are more robust to cold-induced perturbations. The metabolites of cold-hardy flies were less cold responsive and their metabolic networks during cold exposure were more robust, supporting our hypotheses. Metabolites involved in membrane lipid synthesis, tryptophan metabolism, oxidative stress, energy balance, and proline metabolism were altered by selection on cold tolerance. We discuss the potential significance of these alterations.
- Subjects
COLD adaptation; ROBUST control; DROSOPHILA melanogaster; METABOLISM; COLD-blooded animals; PHYSIOLOGICAL effects of temperature
- Publication
Evolution, 2014, Vol 68, Issue 12, p3505
- ISSN
0014-3820
- Publication type
Article
- DOI
10.1111/evo.12541