We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Multimodal nonlinear microscopy: A powerful label-free method for supporting standard diagnostics on biological tissues.
- Authors
Cicchi, Riccardo; Pavone, Francesco Saverio
- Abstract
The large use of nonlinear laser scanning microscopy in the past decade paved the way for potential clinical application of this imaging technique. Modern nonlinear microscopy techniques offer promising label-free solutions to improve diagnostic performances on tissues. In particular, the combination of multiple nonlinear imaging techniques in the same microscope allows integrating morphological with functional information in a morpho-functional scheme. Such approach provides a high-resolution label-free alternative to both histological and immunohistochemical examination of tissues and is becoming increasingly popular among the clinical community. Nevertheless, several technical improvements, including automatic scanning and image analysis, are required before the technique represents a standard diagnostic method. In this review paper, we highlight the capabilities of multimodal nonlinear microscopy for tissue imaging, by providing various examples on colon, arterial and skin tissues. The comparison between images acquired using multimodal nonlinear microscopy and histology shows a good agreement between the two methods. The results demonstrate that multimodal nonlinear microscopy is a powerful label-free alternative to standard histopathological methods and has the potential to find a stable place in the clinical setting in the near future.
- Subjects
NONLINEAR imaging sensors; OPTICAL imaging sensors; IMAGE analysis; NONLINEAR analysis; NONLINEAR statistical models; MATHEMATICAL analysis; HISTOLOGY
- Publication
Journal of Innovative Optical Health Sciences, 2014, Vol 7, Issue 5, p-1
- ISSN
1793-5458
- Publication type
Article
- DOI
10.1142/S1793545813300085