We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Identification and Characterization of the Insecticidal Toxin "Makes Caterpillars Floppy" in Photorhabdus temperata M1021 Using a Cosmid Library.
- Authors
Ullah, Ihsan; Eun-Kyung Jang; Min-Sung Kim; Jin-Ho Shin; Gun-Seok Park; Khan, Abdur Rahim; Sung-Jun Hong; Byung-Kwon Jung; JungBae Choi; YeongJun Park; Yunyoung Kwak; Jae-Ho Shin
- Abstract
Photorhabdus temperata is an entomopathogenic enterobacterium; it is a nematode symbiont that possesses pathogenicity islands involved in insect virulence. Herein, we constructed a P. temperata M1021 cosmid library in Escherichia coli XL1-Blue MRF' and obtained 7.14 × 105 clones. However, only 1020 physiologically active clones were screened for insect virulence factors by injection of each E. coli cosmid clone into Galleria mellonella and Tenebrio molitor larvae. A single cosmid clone, PtC1015, was consequently selected due to its characteristic virulent properties, e.g., loss of body turgor followed by death of larvae when the clone was injected into the hemocoel. The sequence alignment against the available sequences in Swiss-Prot and NCBI databases, confirmed the presence of the mcf gene homolog in the genome of P. temperata M1021 showing 85% homology and 98% query coverage with the P. luminescens counterpart. Furthermore, a 2932 amino acid long Mcf protein revealed limited similarity with three protein domains. The N-terminus of the Mcf encompassed consensus sequence for a BH3 domain, the central region revealed similarity to toxin B, and the C-terminus of Mcf revealed similarity to the bacterial export domain of ApxIVA, an RTX-like toxin. In short, the Mcf toxin is likely to play a role in the elimination of insect pests, making it a promising model for use in the agricultural field.
- Subjects
TOXINS; CATERPILLARS; INSECTICIDAL plants; MICROBIAL virulence; COSMIDS; GREATER wax moth; TENEBRIO molitor; INSECT pests
- Publication
Toxins, 2014, Vol 6, Issue 7, p2024
- ISSN
2072-6651
- Publication type
Article
- DOI
10.3390/toxins6072024