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- Title
EFFECT OF TEMPERATURE ON THE VIABILITY, LIFESPAN AND VIGOUR, OF CHLOROPHYLLOUS SPORES OF OSMUNDASTRUM CINNAMOMEUM (OSMUNDACEAE).
- Authors
L. CHANG; W. L. CHIOU; Y. M. HUANG, Y. M.
- Abstract
Short-lived chlorophyllous spores need special treatment to extend their longevity and vigour for ex situ conservation. Germination rates, mean longevity and germination times of chlorophyllous spores of the rare fern Osmundastrum cinnamomeum were determined to assess their viability, lifespan and vigour at 18--25°C (room temperature, RT), 4°C and -80°C during 96 weeks of storage. Results showed that at the low temperatures, the viability and vigour were significantly maintained, and the lifespan of O. cinnamomeum spores was extended. Spores stored at RT had completely lost all ability to germinate within 12 weeks. Some spores retained their viability for more than 96 weeks at 4°C and -80°C. Mean longevities were 6.1, 41.8, and >96 weeks with storage at RT, 4°C and -80°C respectively. The mean spore germination time (MGT), herein regarded as an indicator of vigour, was affected by both storage temperature and time. Spores stored at RT and 4°C began to lose their vigour after three weeks. However, it did not decline at -80°C until 24 weeks of storage. This study demonstrates that low temperature (4°C) and cryopreservation (-80°C) significantly extended the longevity and vigour of chlorophyllous spores of O. cinnamomeum.
- Subjects
CRYOPRESERVATION of plant cells &; tissues; GERMINATION; FERNS; INTRODUCED species; PLANT species; PLANT spores
- Publication
Fern Gazette, 2015, Vol 20, Issue 2, p55
- ISSN
0308-0838
- Publication type
Article