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- Title
Neuroprotective effects of Apigenin in Human Neuroblastoma SH-SY5Y cells Against Rotenone toxicity: a pilot study.
- Authors
Anusha, Chandran; Sumathi, Thangarajan; Rajkumar, Mayan; Kaveri, Krishnaswamy
- Abstract
Parkinson's disease (PD) is a degenerative disorder of the central nervous system that often impairs the sufferer's motor skills, speech, and other functions. It is a debilitating neurodegenerative disorder characterized by the loss of dopaminergic neurons in the substantia nigra. The purpose of this study was to investigate the neuroprotective effects of apigenin (AGN) on rotenone (ROT) induced apoptosis in human dopaminergic SH-SY5Y cells. SH-SY5Y cells exposed to the ROT (10μM) significantly decreased the viability of SH-SY5Y cells, when examined by MTT assay and the cytotoxicity was examined by the lactate dehydrogenase released into the culture medium. DNA fragmentation was assayed as index of apoptosis. The nuclear damage and the cellular morphology were assessed by a fluorescent DNA-binding probe Hoechst 33258 and phase contrast microscopy, respectively. The results indicated that AGN increased the cell viability (MTT reduction), prevented the membrane damage (LDH release), in ROT induced SH-SY5Y cells. DNA laddering produced by ROT treatment was also prevented by apigenin. Apigenin pre-treatment attenuated the ROT induced cell shrinkage and nuclear condensation. These findings demonstrate that the flavonoid apigenin have neuroprotective effects against ROT induced neurotoxicity in SH-SY5Y cells and might provide a useful therapeutic strategy for the treatment of neurodegenerative disease like Parkinson's disease.
- Subjects
PARKINSON'S disease treatment; NEUROPROTECTIVE agents; APIGENIN; NEUROBLASTOMA; ROTENONE; DRUG toxicity; PILOT projects; MOTOR ability; THERAPEUTICS
- Publication
Drug Invention Today, 2014, Vol 6, Issue 1, p6
- ISSN
0975-7619
- Publication type
Article