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- Title
Four cleaved amplified polymorphic sequence (CAPS) markers for the detection of the Juglans ailantifolia chloroplast in putatively native J. cinerea populations.
- Authors
MCCLEARY, TIM S.; ROBICHAUD, RODNEY L.; NUANES, STEVE; ANAGNOSTAKIS, SANDRA L.; SCHLARBAUM, SCOTT E.; ROMERO-SEVERSON, JEANNE
- Abstract
Hybridization between butternut ( Juglans cinerea), a forest tree native to eastern North America, and Japanese walnut ( J. ailantifolia), a tree tolerant to the lethal fungal disease butternut canker, casts doubt on the genetic identity of the remaining butternuts. We report a diagnostic test to distinguish the J. cinerea chloroplast from the J. ailantifolia chloroplast using cleaved amplified polymorphic sequences resolvable in 1.5% agarose gels. J. ailantifolia maternal ancestry in naturally regenerated stands provides a site selection criterion for studies of introgression dynamics when the non-native parent and the hybrids tolerate a disease to which the native species is susceptible.
- Subjects
BUTTERNUT (Tree); SPECIES hybridization; WALNUT; FUNGAL diseases of plants; CHLOROPLASTS; GENETIC polymorphisms; PLANT species
- Publication
Molecular Ecology Resources, 2009, Vol 9, Issue 2, p525
- ISSN
1755-098X
- Publication type
Article
- DOI
10.1111/j.1755-0998.2008.02465.x