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- Title
Impact of genetic polymorphisms of four cytokine genes on treatment induced viral clearance in HCV infected Egyptian patients.
- Authors
Obada, Manar; El-Fert, Ashraf; Hashim, Mohamed S.; Obada, Mones; Ehsan, Nermin; Alhadad, Omkolsoum; El-Said, Hala
- Abstract
Background: Many factors contribute for viral clearance and response to antiviral therapy. Genetic polymorphisms of cytokines, chemokines, and their receptors can alter the immune response against Hepatitis C virus (HCV). Aim of the study: The aim of the current study is to assess single nucleotide polymorphism (SNP) in the promoter region of IL-10, TNF-α, IFN-ώ and TGF-β as predictors of response to combined Pegylated interferon a/ribavirin (PEG-IFN/RBV) therapy in chronic HCV infected Egyptian patients. Patients and methods: The study was conducted on 150 HCV infected patients and 100 apparently healthy control subjects. All patients were treated with PEG-IFN/RBV. They were classified according to their response to treatment. Genotyping of IL-10, TNF-α, IFN-ώ and TGF-β were performed on peripheral blood DNA using polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) and primer specific assays. Results: Overall, 83/150 (55.3%) patients achieved sustained virological response (SVR), whereas 67 (44.7%) did not. Age and BMI were significantly lower in patients who achieved SVR (P<0.05). IL-10 at site (±1082) GG genotype was associated with SVR where odds ratio was 1.98 with 95% confidence interval (1.34-3.65). None of the other genes showed a significant association with SVR. Conclusion: Analysis of IL-10 SNP at promoter site (±1082) could be used as a pretreatment predictor of response to combined PEG-IFN/RBV treatment.
- Subjects
DOMINANCE (Genetics); MOLECULAR genetics; HEREDITY; GENES; HIV-positive persons
- Publication
Egyptian Journal of Medical Human Genetics, 2017, Vol 18, Issue 2, p111
- ISSN
1110-8630
- Publication type
Article
- DOI
10.1016/j.ejmhg.2016.04.002