We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Syringic acid ameliorates ischemia/reperfusion-induced testicular injury in rats via suppressing of HMGB1/NF-κB axis and endoplasmic reticulum stress.
- Authors
Demir, Elif Ayazoglu; Demir, Selim; Kazaz, Ilke Onur; Kucuk, Hatice; Alemdar, Nihal Turkmen; Gecici, Omer Faruk; Mentese, Ahmet; Aliyazicioglu, Yuksel
- Abstract
Purpose: To investigate the possible protective role of syringic acid on torsion/detorsion-induced testicular injury using biochemical and histopathological approaches for the first time. Methods: A total of 24 rats were divided into 4 groups: sham control, torsion/detorsion, torsion/detorsion + syringic acid (50 mg/kg and 100 mg/kg). Tissue malondialdehyde, total oxidant status and total antioxidant status levels were determined using colorimetric methods. Tissue 8-hydroxy-2′-deoxyguanosine, superoxide dismutase, catalase, high mobility group box 1, nuclear factor kappa B protein 65, tumor necrosis factor-alpha, interleukin-6, myeloperoxidase, 78-kDa glucose-regulated protein, activating transcription factor-6, C/EBP homologous protein and caspase-3 levels were determined using commercial enzyme-linked immunosorbent assay kits. Johnsen's testicle scoring system was used for histological evaluation. Results: Compared with the control group, the levels of oxidative stress, inflammation, endoplasmic reticulum stress and apoptosis were significantly increased in the torsion/detorsion group (p < 0.05). Syringic acid administrations statistically significantly restored these damage in a dose dependent manner (p < 0.05). Moreover, it was found that the results of histological examinations supported the biochemical results to a statistically significant extent. Conclusion: The overall results suggest that syringic acid emerges as a potential compound for the treatment of testicular torsion and may be subject to clinical trials.
- Subjects
TESTIS injuries; ISCHEMIA prevention; INTERLEUKINS; KRUSKAL-Wallis Test; ENDOPLASMIC reticulum; INFLAMMATION; ANIMAL experimentation; OXIDIZING agents; ANTIOXIDANTS; SUPEROXIDE dismutase; NF-kappa B; APOPTOSIS; HEALTH outcome assessment; MANN Whitney U Test; OXIDATIVE stress; RATS; MALONDIALDEHYDE; CATALASE; TUMOR necrosis factors; ENZYME-linked immunosorbent assay; DESCRIPTIVE statistics; PLANT extracts; COLORIMETRY; TRANSCRIPTION factors; DATA analysis software; REPERFUSION injury; CASPASES
- Publication
European Journal of Trauma & Emergency Surgery, 2023, Vol 49, Issue 3, p1595
- ISSN
1863-9933
- Publication type
Article
- DOI
10.1007/s00068-023-02227-7