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- Title
Long Non-Coding Ribonucleic Acid PGM5-AS1 Regulates Lung Cancer Progression by Modulating microrna-587 to Affect Cell Ferroptosis.
- Authors
Jian Zhong; Jie Jiang; Lei Cao; Zicheng Liu; Bo Wang
- Abstract
The objective of this study was to analyze the influence of long non-coding ribonucleic acid PGM5-AS1 on lung cancer cell ferroptosis through microrna-587. To this end, the research team has included the abnormal expression vectors of PGM5-AS1 and microrna-587 were transfected into human lung cancer cell line A549 to detect alterations in cell activity, apoptosis, cell cycle, oxidative stress, iron ion and ferroptosis-associated proteins. The results show silencing PGM5-AS1 or elevating microrna-587 promoted A549 proliferation and invasion, while inhibiting apoptosis and resisting ferroptosis. However, increasing PGM5-AS1 or silencing microrna-587 enhanced A549 apoptosis and induced ferroptosis (P < 0.05). And in the salvage experiment, the research team found that microrna-587 mimics inhibited PGM5-AS1-wild sequence fluorescence activity. The biological behavior of A549 after increasing (or silencing) PGM5-AS1 and microrna-587 was not different from that of cells transfected with microrna-587 negative control (P > 0.05). These results suggest that lowexpressed PGM5-AS1 can promote lung cancer cell viability, inhibit apoptosis and resist ferroptosis through targeted increase of microrna-587.
- Subjects
RNA physiology; DISEASE progression; REVERSE transcriptase polymerase chain reaction; FLOW cytometry; WESTERN immunoblotting; LUNG tumors; DESCRIPTIVE statistics; CELL lines; DATA analysis software; CELL death
- Publication
Current Topics in Nutraceutical Research, 2024, Vol 22, Issue 1, p213
- ISSN
1540-7535
- Publication type
Article
- DOI
10.37290/ctnr2641-452X.22213-221