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- Title
Rapid healing of femoral defects in rats with low dose sustained BMP2 expression from PEGDA hydrogel microspheres.
- Authors
Sonnet, Corinne; Simpson, C. LaShan; Olabisi, Ronke M.; Sullivan, Kayleigh; Lazard, ZaWaunyka; Gugala, Zbigniew; Peroni, John F.; Weh, J. Michael; Davis, Alan R.; West, Jennifer L.; Olmsted ‐ Davis, Elizabeth A.
- Abstract
ABSTRACT Current strategies for bone regeneration after traumatic injury often fail to provide adequate healing and integration. Here, we combined the poly (ethylene glycol) diacrylate (PEGDA) hydrogel with allogeneic 'carrier' cells transduced with an adenovirus expressing BMP2. The system is unique in that the biomaterial encapsulates the cells, shielding them and thus suppressing destructive inflammatory processes. Using this system, complete healing of a 5 mm-long femur defect in a rat model occurs in under 3 weeks, through secretion of 100-fold lower levels of protein as compared to doses of recombinant BMP2 protein used in studies which lead to healing in 2-3 months. New bone formation was evaluated radiographically, histologically, and biomechanically at 2, 3, 6, 9, and 12 weeks after surgery. Rapid bone formation bridged the defect area and reliably integrated into the adjacent skeletal bone as early as 2 weeks. At 3 weeks, biomechanical analysis showed the new bone to possess 79% of torsional strength of the intact contralateral femur. Histological evaluation showed normal bone healing, with no infiltration of inflammatory cells with the bone being stable approximately 1 year later. We propose that these osteoinductive microspheres offer a more efficacious and safer clinical option over the use of rhBMP2. © 2013 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 31:1597-1604, 2013
- Subjects
WOUND healing; FEMUR injuries; DRUG dosage; BONE morphogenetic proteins; GENE expression; MICROSPHERES; COLLOIDS in medicine; LABORATORY rats
- Publication
Journal of Orthopaedic Research, 2013, Vol 31, Issue 10, p1597
- ISSN
0736-0266
- Publication type
Article
- DOI
10.1002/jor.22407