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- Title
Fully automated processing of buffy-coat–derived pooled platelet concentrates.
- Authors
Janetzko, Karin; Klüter, Harald; Van Waeg, Geert; Eichler, Hermann
- Abstract
The OrbiSac device, which was develo-ped to automate the manufacture of buffy-coat PLT con-centrates (BC-PCs), was evaluated. In-vitro characteristics of BC-PC preparations using the OrbiSac device were compared with manually prepared BC-PCs. For standard processing (Std-PC, n = 20), four BC-PCs were pooled using 300 mL of PLT AS (PAS) followed by soft-spin centrifugation and WBC filtration. The OrbiSac pre-paration (OS-PC, n = 20) was performed by automated pooling of four BC-PCs with 300 mL PAS followed by centrifugation and inline WBC filtration. All PCs were stored at 22°C. Samples were withdrawn on Day 1, 5, and 7 evaluating PTL count, blood gas analysis, glucose, lactate, LDH, β-thromboglobulin, hypotonic shock response, and CD62p expression. A PLT content of 3.1 ± 0.4 × 1011 (OS-PCs) versus 2.7 ± 0.5 × 1011 (Std-PCs, p < 0.05) was found. A CV of 19 percent (Std-PC) versus 14 percent (OS-PC) suggests more standardization in the OS group. At Day 7, the Std-PCs versus OS-PCs showed a glucose consumption of 1.03 ± 0.32 µmol per 109 PLT versus 0.75 ± 0.25 µmol per 109 PLT (p < 0.001), and a lactate production of 1.50 ± 0.86 µmol per 109 versus 1.11 ± 0.61 µmol per 109 (p < 0.001). The pH (7.00 ± 0.19 vs. 7.23 ± 0.06; p < 0.001), pO2 (45.3 ± 18 vs. 31.3 ± 10.4 mmHg; p < 0.01), and HCO3 levels (4.91 ± 1.49 vs. 7.14 ± 0.95 mmol/L; p < 0.001) suggest a slightly better aerobic metabolism within the OS group. Only small differences in CD62p expression was observed (37.3 ± 12.9% Std-PC vs. 44.8 ± 6.6% OS-PC; p < 0.05). The OrbiSac device allows an improved PLT yield without affecting PLT in-vitro characteristics and may enable an improved consistency in product volume and yield.
- Subjects
BLOOD platelets; LEUCOCYTES; BLOOD filtration; BLOOD gases analysis; GLUCOSE; BLOOD lactate
- Publication
Transfusion, 2004, Vol 44, Issue 7, p1052
- ISSN
0041-1132
- Publication type
Article
- DOI
10.1111/j.1537-2995.2004.03296.x