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- Title
Simultaneous detection of imidacloprid and parathion by the dual-labeled time-resolved fluoroimmunoassay.
- Authors
Shi, Haiyan; Sheng, Enze; Feng, Lu; Zhou, Liangliang; Hua, Xiude; Wang, Minghua
- Abstract
A highly sensitive direct dual-labeled time-resolved fluoroimmunoassay (TRFIA) to detect parathion and imidacloprid simultaneously in food and environmental matrices was developed. Europium (Eu) and samarium (Sm) were used as fluorescent labels by coupling separately with L-Ab and AP-Ab. Under optimal assay conditions, the half-maximal inhibition concentration (IC) and limit of detection (LOD, IC) were 10.87 and 0.025 μg/L for parathion and 7.08 and 0.028 μg/L for imidacloprid, respectively. The cross-reactivities (CR) were negligible except for methyl-parathion (42.4 %) and imidaclothiz (103.4 %). The average recoveries of imidacloprid ranged from 78.9 to 104.2 % in water, soil, rice, tomato, and Chinese cabbage with a relative standard deviation (RSD) of 2.4 to 11.6 %, and those of parathion were from 81.5 to 110.9 % with the RSD of 3.2 to 10.5 %. The results of TRFIA for the authentic samples were validated by comparison with gas chromatography (GC) analyses, and satisfactory correlations (parathion: R = 0.9918; imidacloprid: R = 0.9908) were obtained. The results indicate that the dual-labeled TRFIA is convenient and reliable to detect parathion and imidacloprid simultaneously in food and environmental matrices.
- Subjects
IMIDACLOPRID; PARATHION; SAMARIUM compounds; CHOLINESTERASE-inhibiting insecticides; FLUORESCENCE polarization immunoassay; PHYSIOLOGY
- Publication
Environmental Science & Pollution Research, 2015, Vol 22, Issue 19, p14882
- ISSN
0944-1344
- Publication type
Article
- DOI
10.1007/s11356-015-4697-y