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- Title
Efficient CNS gene delivery by intravenous injection.
- Authors
Louboutin, Jean-Pierre; Chekmasova, Alena A.; Marusich, Elena; Chowdhury, J. Roy; Strayer, David S.
- Abstract
We administered recombinant SV40-derived viral vectors (rSV40s) intravenously to mice with or without prior intraperitoneal injection of mannitol to deliver transgenes to the central nervous system (CNS). We detected transgene-expressing cells (mainly neurons) most prominently in the cortex and spinal cord; prior intraperitoneal mannitol injection increased CNS gene delivery tenfold. Intravenous injection of rSV40s, particularly with mannitol pretreatment, resulted in extensive expression of multiple transgenes throughout the CNS.
- Subjects
SV40 (Virus); CENTRAL nervous system; INTRAVENOUS injections; MANNITOL; TRANSGENES
- Publication
Nature Methods, 2010, Vol 7, Issue 11, p905
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1518