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- Title
Single-gene transgenic mouse strains for reprogramming adult somatic cells.
- Authors
Carey, Bryce W.; Markoulaki, Styliani; Beard, Caroline; Hanna, Jacob; Jaenisch, Rudolf
- Abstract
We report transgenic mouse models in which three or four reprogramming factors are expressed from a single genomic locus using a drug-inducible transgene. Multiple somatic cell types can be directly reprogrammed to generate induced pluripotent stem cells (iPSCs) by culture in doxycycline. Because reprogramming factors are carried on a single polycistronic construct, the mice can be easily maintained, and the transgene can be easily transferred into other genetic backgrounds.
- Subjects
TRANSGENIC mice; GENOMES; TRANSGENES; SOMATIC cells; STEM cells
- Publication
Nature Methods, 2010, Vol 7, Issue 1, p56
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1410