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- Title
Interleukin 27 polymorphisms in HCV RNA positive patients: is there an impact on response to interferon therapy?
- Authors
Zicca, Emilia; Quirino, Angela; Marascio, Nadia; Nucara, Stefania; Fabiani, Fernanda; Trapasso, Francesco; Perrotti, Nicola; Strazzulla, Alessio; Torti, Carlo; Liberto, Maria; Focà, Alfredo; Liberto, Maria Carla
- Abstract
<bold>Background: </bold>Interleukin 27 (IL-27) has pleiotropic properties that can either limit or enhance immune responses. Recent studies revealed that single nucleotide polymorphisms (SNPs) of the IL-27 promoter region modulate the development of infectious diseases and individual's susceptibility to therapeutic response. Little is known about the relationship between IL-27 single nucleotide polymorphisms and therapy response in patients infected by hepatitis C virus (HCV). In this study we have investigated the potential role of SNPs in the promoter region of IL27 p28 gene (alleles rs153109) on the outcome of HCV infected patients.<bold>Methods: </bold>rs153109, corresponding to position c.-964A>G of the IL-27 locus, was amplified from genomic DNA extracted from 15 patients with chronic hepatitis C stratified by sustained viral response (SVR), relapser and non-responder, after treatment with peginterferon-α (PegIFN- α) combined with ribavirin (RBV). Amplification products were studied by direct sequencing.<bold>Results: </bold>This method has been applied in a preliminary study on patients with chronic hepatitis C to provide information for a standardized assay useful to genotyping of rs153109 SNPs of IL-27p28. The genotype distribution of the c.-964 A>G polymorphism was more present in patients who did not achieve a SVR. By contrast, the genotype G/G was absent in non-responder and relapser patients. Moreover, the analysis of allelic distribution of rs153109 highlighted a predominance of allele A in all genotypes in spite of allele G.<bold>Conclusions: </bold>Our work provides preliminary information for a standardized method potentially useful for genotyping rs153109, and suggests its utility as a candidate approach to evaluate IL-27 p28 polymorphisms as additional clinical predictors of response to therapies in HCV infected patients.
- Publication
BMC Infectious Diseases, 2014, Vol 14, pS5
- ISSN
1471-2334
- Publication type
journal article
- DOI
10.1186/1471-2334-14-S5-S5