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- Title
Conversion of 11-hydroxy- O-methylsterigmatocystin to aflatoxin G in Aspergillus parasiticus.
- Authors
Hongmei Zeng; Hatabayashi, Hidemi; Nakagawa, Hiroyuki; Jingjing Cai; Suzuki, Ryoya; Sakuno, Emi; Tanaka, Toshitsugu; Ito, Yasuhiro; Ehrlich, Kenneth C.; Nakajima, Hiromitsu; Yabe, Kimiko
- Abstract
In aflatoxin biosynthesis, aflatoxins G (AFG) and B (AFB) are independently produced from a common precursor, O-methylsterigmatocystin (OMST). Recently, 11-hydroxy -O-methylsterigmatocystin (HOMST) was suggested to be a later precursor involved in the conversion of OMST to AFB, and conversion of HOMST to AFB was catalyzed by OrdA enzyme. However, the involvement of HOMST in AFG formation has not been determined. In this work, HOMST was prepared by incubating OrdA-expressing yeast with OMST. Feeding Aspergillus parasiticus with HOMST allowed production of AFG as well as AFB. In cell-free systems, HOMST was converted to AFG when the microsomal fraction, the cytosolic fraction from A. parasiticus, and yeast expressing A. parasiticus OrdA were added. These results demonstrated (1) HOMST is produced from OMST by OrdA, (2) HOMST is a precursor of AFG as well as AFB, and (3) three enzymes, OrdA, CypA, and NadA, and possibly other unknown enzymes are involved in conversion of HOMST to AFG.
- Subjects
ASPERGILLUS flavus; AFLATOXINS; BIOSYNTHESIS; YEAST fungi biotechnology; ENZYMES; CHEMICAL synthesis; FOOD contamination
- Publication
Applied Microbiology & Biotechnology, 2011, Vol 90, Issue 2, p635
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-010-2999-z