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- Title
O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104.
- Authors
Zdorovenko, E. L.; Wang, Y.; Shashkov, A. S.; Chen, T.; Ovchinnikova, O. G.; Liu, B.; Golomidova, A. K.; Babenko, V. V.; Letarov, A. V.; Knirel, Y. A.
- Abstract
Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces. The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF were studied by monosaccharide analysis and one- and two-dimensional 1H- and 13C-NMR spectroscopy. Both OPSs had similar structures and differed only in the presence of a side-chain glucose residue in the strain HS3-104 OPS. The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.
- Subjects
O antigens; ESCHERICHIA coli; GLYCEROLPHOSPHATE dehydrogenase; POLYSACCHARIDES; OLIGOSACCHARIDES
- Publication
Biochemistry (00062979), 2018, Vol 83, Issue 5, p534
- ISSN
0006-2979
- Publication type
Article
- DOI
10.1134/S0006297918050061