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- Title
Real-Time Fluorescence Imaging Using Indocyanine Green to Assess Therapeutic Effects of Near-Infrared Photoimmunotherapy in Tumor Model Mice.
- Authors
Rosenberg, Adrian; Fujimura, Daiki; Okada, Ryuhei; Furusawa, Aki; Inagaki, Fuyuki; Wakiyama, Hiroaki; Kato, Takuya; Choyke, Peter L.; Kobayashi, Hisataka
- Abstract
Background: Near-infrared photoimmunotherapy (NIR-PIT) is a cancer therapy that causes an increase in tumor perfusion, a phenomenon termed the super-enhanced permeability and retention effect. Currently, in vivo treatment efficacy of NIR-PIT is observable days after treatment, but monitoring would be improved by more acute detection of intratumor change. Fluorescence imaging may detect increased tumor perfusion immediately after treatment. Methods: In the first experiment, athymic nude mouse models bearing unilateral subcutaneous flank tumors were treated with either NIR-PIT or laser therapy only. In the second experiment, mice bearing bilateral flank tumors were treated with NIR-PIT only on the left-sided tumor. In both groups, immediately after treatment, indocyanine green was injected at different doses intravenously, and mice were monitored with the Shimadzu LIGHTVISION fluorescence imaging system for 1 hour. Results: Tumor-to-background ratio of fluorescence intensity increased over the 60 minutes of monitoring in treated mice but did not vary significantly in control mice. Tumor-to-background ratio was highest in the 1 mg kg−1 and 0.3 mg kg−1 doses. In mice with bilateral tumors, tumor-to-untreated tumor ratio increased similarly. Conclusions: Acute changes in tumor perfusion after NIR-PIT can be detected by real-time fluorescence imaging.
- Subjects
TREATMENT effectiveness; INDOCYANINE green; MICE; IMAGING systems; TUMORS; MAGNETIC resonance angiography; FLUORESCENCE
- Publication
Molecular Imaging, 2020, Vol 19, p1
- ISSN
1535-3508
- Publication type
Article
- DOI
10.1177/1536012120934965