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- Title
Membrane localization of the MAK-V protein kinase.
- Authors
Kalinichenko, S. V.; Korobko, E. V.; Korobko, I. V.
- Abstract
Activities of many proteins including protein kinases are often regulated by their dynamic association with specific intracellular compartments. MAK-V is an AMPK-like protein kinase with poorly characterized functions and mechanisms of action. Similarly to many other protein kinases, association of MAK-V with specific intracellular compartments could be essential for its proper functions. In this work, we studied subcellular distribution of exogenously produced and endogenous MAK-V proteins in mammalian cells using biochemical cell fractioning aiming to supplement data on MAK-V intracellular localization studied by immunocytochemical methods. We found that a significant portion of MAK-V protein in mammalian cells is associated with membranes. Moreover, MAK-V expressed in yeast was also targeted to membrane, thus suggesting an evolutionarily conservative mechanism of MAK-V membrane association. Based on the ability of various MAK-V deletion mutants to localize to membrane and comparison of MAK-V amino acid sequences from different species, we suggest a possible mechanism governing MAK-V association with intracellular membranes.
- Subjects
PROTEIN analysis; BIOMOLECULES; PROTEIN kinases; PHOSPHOTRANSFERASES; ORGANIC acids; AMINO acid sequence
- Publication
Biochemistry (00062979), 2008, Vol 73, Issue 3, p278
- ISSN
0006-2979
- Publication type
Article
- DOI
10.1134/S0006297908030061