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- Title
Improving biobutanol production in engineered Saccharomyces cerevisiae by manipulation of acetyl-CoA metabolism.
- Authors
Krivoruchko, Anastasia; Serrano-Amatriain, Cristina; Chen, Yun; Siewers, Verena; Nielsen, Jens
- Abstract
Recently, butanols (1-butanol, 2-butanol and iso-butanol) have generated attention as alternative gasoline additives. Butanols have several properties favorable in comparison to ethanol, and strong interest therefore exists in the reconstruction of the 1-butanol pathway in commonly used industrial microorganisms. In the present study, the biosynthetic pathway for 1-butanol production was reconstructed in the yeast Saccharomyces cerevisiae. In addition to introducing heterologous enzymes for butanol production, we engineered yeast to have increased flux toward cytosolic acetyl-CoA, the precursor metabolite for 1-butanol biosynthesis. This was done through introduction of a plasmid-containing genes for alcohol dehydrogenase ( ADH2), acetaldehyde dehydrogenase ( ALD6), acetyl-CoA synthetase (ACS), and acetyl-CoA acetyltransferase ( ERG10), as well as the use of strains containing deletions in the malate synthase ( MLS1) or citrate synthase ( CIT2) genes. Our results show a trend to increased butanol production in strains engineered for increased cytosolic acetyl-CoA levels, with the best-producing strains having maximal butanol titers of 16.3 mg/l. This represents a 6.5-fold improvement in butanol titers compared to previous values reported for yeast and demonstrates the importance of an improved cytosolic acetyl-CoA supply for heterologous butanol production by this organism.
- Subjects
BIOBUTANOL; SACCHAROMYCES cerevisiae; ACETYLCOENZYME A; METABOLIC regulation; GASOLINE additives; INDUSTRIAL microorganisms; BIOSYNTHESIS
- Publication
Journal of Industrial Microbiology & Biotechnology, 2013, Vol 40, Issue 9, p1051
- ISSN
1367-5435
- Publication type
Article
- DOI
10.1007/s10295-013-1296-0