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- Title
Identificación molecular y diversidad genética de las especies de nematodos Globodera rostochiensis y G. pallida en regiones productoras de papa de Guatemala.
- Authors
Ruiz-Chután, José A.; Berdúo-Sandoval, Julio E.; Melgar, Sergio; Kalousová, Marie; Lojka, Bohdan; Fernández, Eloy; Žiarovská, Jana; Sánchez-Pérez, Amílcar
- Abstract
In Guatemala, potato crop production is affected by the nematodes Globodera rostochiensis and Globodera pallida. The ability of both species to form cysts complicates their control and causes an increase in their populations. In Guatemala, both species of nematodes have been reported by morphological identification; however, molecular confirmation has not been carried out. It is the first study to validate the presence of both nematode species by multiplex PCR and determine the diversity and genetic structure of the populations using molecular markers. Sampling was carried out in four potato-producing departments of the country. PCR identification was performed with the common primer ITS5 and the primers PITSr3 specific for G. rostochiensis and PITSp4 for G. pallida. We performed molecular characterization with the AFLP marker. We confirmed the presence of the two nematode species in the four departments. Shannon diversity and expected heterozygosity indices revealed higher genetic diversity in G. rostochiensis (H = 0.311, He = 0.301) than in G. pallida (H = 0.035, He = 0.223). The NJ, DAPC, and PCA methods exhibited weak structure among populations of both nematode species. The results suggest a dispersal pattern from Quetzaltenango to the rest of the country, attributed to the commercialization of seed contaminated with nematodes. We suggest promoting socialization programs on the benefits of using certified seeds and constant molecular monitoring for an accurate diagnosis of both species of nematodes.
- Subjects
GUATEMALA; GOLDEN nematode; GLOBODERA pallida; GENETIC variation; AGRICULTURAL productivity; AMPLIFIED fragment length polymorphism; POTATOES
- Publication
Ciencia, Tecnología y Salud, 2022, Vol 9, Issue 2, p166
- ISSN
2409-3459
- Publication type
Article
- DOI
10.36829/63CTS.v9i2.930