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- Title
Single‐Cell Profiling of Bone Marrow B Cells and Early B Cell Developmental Disorders Associated With Systemic Lupus Erythematosus.
- Authors
Dong, Chen; Guo, Yicheng; Chen, Zechuan; Li, Teng; Ji, Juan; Sun, Chi; Li, Jing; Cao, Haixia; Xia, Yunfei; Xue, Zhonghui; Gu, Xixi; Liang, Qian; Zhao, Rui; Fu, Ting; Ma, Jiaqiang; Jiang, Shan; Wu, Chunmei; Fu, Qiong; Guo, Genkai; Bao, Yanfeng
- Abstract
Objective: The peripheral B cell compartment is heavily disturbed in systemic lupus erythematosus (SLE), but whether B cells develop aberrantly in the bone marrow (BM) is largely unknown. Methods: We performed single‐cell RNA/B cell receptor (BCR) sequencing and immune profiling of BM B cells and classified patients with SLE into two groups: early B cell (Pro‐B and Pre‐B) normal (EBnor) and EB defective/low (EBlo) groups. Results: The SLE‐EBlo group exhibited more severe disease activity and proinflammatory status, overaction of type I interferon signaling and metabolic pathways within the B cell compartment, and aberrant BCR repertoires compared with the SLE‐EBnor group. Moreover, in one patient with SLE who was initially classified in the SLE‐EBlo group, early B cell deficiency and associated abnormalities were largely rectified in a second BM sample at the remission phase. Conclusion: In summary, this study suggests that early B cell loss in BM defines a unique pathological state in a subset of patients with SLE that may play an active role in the dysregulated autoimmune responses.
- Subjects
TYROSINE metabolism; PHENYLALANINE metabolism; BONE marrow examination; FLOW cytometry; IMMUNOGENETICS; STATISTICAL correlation; BLOOD testing; GENOMICS; CLUSTER analysis (Statistics); PHOSPHORYLATION; QUALITATIVE research; RESEARCH funding; CELL physiology; SYSTEMIC lupus erythematosus; MANN Whitney U Test; GLYCOPROTEINS; QUANTITATIVE research; RNA; INTERFERONS; GENE expression profiling; METADATA; ANALYSIS of variance; METABOLISM; COMPARATIVE studies; COLLECTION &; preservation of biological specimens; CYTOKINES; FACTOR analysis; DATA analysis software; GENETIC mutation; B cells; CELL separation; SEQUENCE analysis; GENETICS; GENOTYPES; DISEASE complications
- Publication
Arthritis & Rheumatology, 2024, Vol 76, Issue 4, p599
- ISSN
2326-5191
- Publication type
Article
- DOI
10.1002/art.42750