We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Diagnostic value of FOXF1 gene promoter-methylated DNA in the plasma samples of patients with colorectal cancer.
- Authors
Dastafkan, Zahra; Rezvani, Nayebali; Amini, Sabrieh
- Abstract
Background: Epigenetic modifications such as DNA methylation in the CpG islands of genes occur at a high rate. In this study, we measured the methylation level of the promoter region of the FOXF1 gene as a new blood biomarker for the detection of colorectal cancer in the early stages. Methods: The methylation level of the promoter region of the FOXF1 gene was measured in the plasma samples of 50 colorectal cancer patients and 50 normal individuals. DNA was extracted after exposure to sodium bisulfite by the MethyLight polymerase chain reaction (PCR) method. The percentage of promoter region was measured in all samples, and statistical analysis was done using SPSS v24 software. Results: The average promoter region between the plasma samples of colorectal cancer patients and healthy individuals had a significant difference (P < 0.001). The average promoter region of the FOXF1 gene in tumor plasma samples was 7.1 and in the control samples was 0.48. The sensitivity and specificity of the sample plasma levels were 78% and 89.5%, respectively. Conclusion: The promoter region value of the FOXF1 gene in plasma samples using the MethyLight PCR method had high sensitivity and specificity as a non-invasive method for colorectal cancer diagnosis. This research is the first report that has been presented regarding the investigation of FOXF1 gene methylation in plasma samples in colorectal cancer. Therefore, it is necessary to conduct more studies with larger size samples to evaluate the efficiency of the gene under investigation.
- Subjects
IRINOTECAN; COLORECTAL cancer; CANCER patients; PROMOTERS (Genetics); DNA; EARLY detection of cancer; METHYLGUANINE
- Publication
International Journal of Biological Markers, 2023, Vol 38, Issue 3/4, p194
- ISSN
0393-6155
- Publication type
Article
- DOI
10.1177/03936155231207109